Cytotoxic effect of cobalt ferrite nanoparticles on NIH-3T3 and malignant cancer cell lines CaCo2 and CAL27

Document Type : Original Research Article


1 Student Research Committee, Arak University of Medical Sciences, Arak, Iran

2 Faculty of Dentistry, Iran University of Medical Sciences, Tehran, Iran


The focus of many investments was pulled towards magnetic nanoparticles and their extending range of implementations in medicine and biology that particularly include drug delivery, magnetic resonance imaging, and cancer treatment through hyperthermia, as well as other objectives such as pollutants degradation and compounds separation. This work attempted to arrange cobalt ferrite nanoparticles (CoFe2O4 NPs) by taking advantage of starch aqueous solution combined with a calcination process at 500, 600, and 700 °C. The acquired prepared NPs, which were muti-dimensional shape, were configured by the employment of X-rày Diffraction (XRD), Vibràting Sample Màgnetometer (VSM) and Energy Dispersive X-rày (EDX), Field-emission Scànning Electron Microscope (FESEM), and Ràman spectroscopy. The outcomes of VSM analysis were indicative of a ferrimagnetic attitude, while the performance of MTT assay helped in assessing the cytotoxicity of CoFe2O4 NPs on colon càncer cell (CaCo2), oral squamous cell carcinoma (OSCC) CAL27 and mouse embryò fibròblast cell (NIH-3T3) lines, which resulted to be non-toxic towards cancer and normal cell lines. Considering these observations, we can approve the ideal applicability of our synthesized CoFe2O4 NPs in medical implementations similar to drug delivery and non-medical utilizations. 


Main Subjects

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