Nanomedicine Research Journal

Nanomedicine Research Journal

Renal cell carcinoma derived exosome as natural nanoparticles inhibits T cell proliferation and induces their apoptosis

Document Type : Original Research Article

Authors
Davood Babakhani 1
Danyal Daneshdoust 2
Amirhossein Baharvand 3
Azam Mivefroshan 4
Iman Raeisi 5
Bashar Zuhair Talib Al-Naqeeb 6
Farnaz Tavakoli 7
1 Assistant Professor of Hematology and Oncology, Hematology, Oncology Research Center and Stem Cell Transplantation (HORCST), Dr. Shariati hospital, Tehran University of Medical Sciences, Tehran Iran
2 Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
3 Student Research Committee, Arak University of Medical Sciences, Arak, Iran
4 Nephrology and renal transplant research center ,urmia university of medical science,urmia , Iran
5 Iman.reeisy@gmail.com
6 Anesthesia technology department, Al-Turath University College , Al Mansour, Baghdad, Iraq
7 Assistant Professor of Nephrology, Tehran University of Medical Sciences, Shariati hospital, Nephrology ward, Tehran, Iran
10.22034/nmrj.2023.03.006
Abstract
Objective(s): To evaluate the anti-tumor effect of the renal cell carcinoma ACHN cell-exosome on human T cell biological parameters.
Methods: Human T cell was isolated from healthy donors. ACHN-cell derived exosome was procured and expression of the CD9 and CD63 was assessed using western blotting. The effects of the ACHN- exosome on T cell proliferation was measured using MTT assay within 24, 48, 72 and 96 hours of treatment with 500-2000 ng/ml exosome. Also, the apoptotic cell percentages in treated cells were assessed by Annexin/PI staining and flowcytometry upon exposure with 500 and 2000 ng/ml exosome within 96 hours of treatment.
Results: According to results, exosome therapy inhibited T cell proliferation more evidently at higher concentration.  Also, treatment with exosome caused an improvement in the apoptosis of the T cell within 96 hours of treatment.
Conclusions: Concerning the results, ACHN- exosome has negative effect on T cell viability and proliferation, facilitating immune evasion.
Keywords
T cell
Exosome
Renal cell carcinoma
Proliferation

Subjects

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Nanomedicine Research Journal
Volume 8, Issue 3
Summer 2023
Pages 277-282