Renal cell carcinoma derived exosome as natural nanoparticles inhibits T cell proliferation and induces their apoptosis

Document Type : Original Research Article


1 Assistant Professor of Hematology and Oncology, Hematology, Oncology Research Center and Stem Cell Transplantation (HORCST), Dr. Shariati hospital, Tehran University of Medical Sciences, Tehran Iran

2 Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran

3 Student Research Committee, Arak University of Medical Sciences, Arak, Iran

4 Nephrology and renal transplant research center ,urmia university of medical science,urmia , Iran


6 Anesthesia technology department, Al-Turath University College , Al Mansour, Baghdad, Iraq

7 Assistant Professor of Nephrology, Tehran University of Medical Sciences, Shariati hospital, Nephrology ward, Tehran, Iran


Objective(s): To evaluate the anti-tumor effect of the renal cell carcinoma ACHN cell-exosome on human T cell biological parameters.
Methods: Human T cell was isolated from healthy donors. ACHN-cell derived exosome was procured and expression of the CD9 and CD63 was assessed using western blotting. The effects of the ACHN- exosome on T cell proliferation was measured using MTT assay within 24, 48, 72 and 96 hours of treatment with 500-2000 ng/ml exosome. Also, the apoptotic cell percentages in treated cells were assessed by Annexin/PI staining and flowcytometry upon exposure with 500 and 2000 ng/ml exosome within 96 hours of treatment.
Results: According to results, exosome therapy inhibited T cell proliferation more evidently at higher concentration.  Also, treatment with exosome caused an improvement in the apoptosis of the T cell within 96 hours of treatment.
Conclusions: Concerning the results, ACHN- exosome has negative effect on T cell viability and proliferation, facilitating immune evasion.


Main Subjects

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