Nanomedicine Research Journal

Nanomedicine Research Journal

A new method for identifying methadone in urine samples by trapping it in dendrimer

Document Type : Original Research Article

Authors
Mayam Tahmasebi 1
Akbar Esmaeili 2
1 Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, Tehran, Iran
10.22034/nmrj.2023.03.010
Abstract
This article used an accurate urine sample (US) from a person with daily use of methadone (MTD), whose presence was confirmed through the diffusion liquid-liquid microextraction method followed by the gas chromatography-mass spectroscopy (GC-MS) technique. At a temperature of 40 0C, keep the sample. The 2nd generation polyamidoamine dendrimer (2-GPD), which was previously synthesized, was released in the US Samples were taken from the container at different intervals.
It injected the model into the high-performance liquid chromatography ultraviolet (HPLC-UV) machine, filtering and extraction by diffusion liquid-liquid microextraction. Examining the HPLC-UV absorption of the samples taken from the US shows that with time MTD molecules migrate from the US into the cavities of the 2-GPD, and the ultraviolet absorption of MTD decreases. The finding confirms the suitability of 2-GPD as an agent for the extraction. 
The identification of MTD was reported in the actual US Monitoring and controlling the consumption of various drugs, such as MTD, is a challenging clinical and legal toxicology issue. Examining medications in a model such as the US always requires high accuracy. These methods include. The pre-purification stage is based on precision instruments. 
Keywords
Methadone
2nd generation polyamidoamine dendrimer
Urine sample
Trapping
High-performance liquid chromatography

Subjects

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Nanomedicine Research Journal
Volume 8, Issue 3
Summer 2023
Pages 311-321