A new method for identifying methadone in urine samples by trapping it in dendrimer

Document Type : Original Research Article


1 Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

2 Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, Tehran, Iran


This article used an accurate urine sample (US) from a person with daily use of methadone (MTD), whose presence was confirmed through the diffusion liquid-liquid microextraction method followed by the gas chromatography-mass spectroscopy (GC-MS) technique. At a temperature of 40 0C, keep the sample. The 2nd generation polyamidoamine dendrimer (2-GPD), which was previously synthesized, was released in the US Samples were taken from the container at different intervals.
It injected the model into the high-performance liquid chromatography ultraviolet (HPLC-UV) machine, filtering and extraction by diffusion liquid-liquid microextraction. Examining the HPLC-UV absorption of the samples taken from the US shows that with time MTD molecules migrate from the US into the cavities of the 2-GPD, and the ultraviolet absorption of MTD decreases. The finding confirms the suitability of 2-GPD as an agent for the extraction. 
The identification of MTD was reported in the actual US Monitoring and controlling the consumption of various drugs, such as MTD, is a challenging clinical and legal toxicology issue. Examining medications in a model such as the US always requires high accuracy. These methods include. The pre-purification stage is based on precision instruments. 


Main Subjects

  1. S.R. Ahmed, R. Chand, S. Kumar, N. Mittal, S. Srinivasan, A.R. Rajabzadeh, Recent biosensing advances in the rapid detection of illicit drugs, TrAC Trends in Analytical Chemistry 131 (2020) 116006. https://doi.org/10.1016/j.trac.2020.116006
  2. K.E. Dunn, P.H. Finan, D.A. Tompkins, E.C. Strain, Frequency and correlates of sleep disturbance in methadone and buprenorphine-maintained patients, Addictive behaviors 76 (2018) 8-14. https://doi.org/10.1016/j.addbeh.2017.07.016  
  3. J. Strang, N.D. Volkow, L. Degenhardt, M. Hickman, K. Johnson, G.F. Koob, B.D. Marshall, M. Tyndall, S.L. Walsh, Opioid use disorder, Nature reviews Disease primers 6(1) (2020) 1-28. https://doi.org/10.1038/s41572-019-0137-5  
  4. T. Vos, A.A. Abajobir, K.H. Abate, C. Abbafati, K.M. Abbas, F. Abd-Allah, R.S. Abdulkader, A.M. Abdulle, T.A. Abebo, S.F. Abera, Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016, The Lancet 390(10100) (2017) 1211-1259.  
  5. S. Masoumi, A. Esmaeili, New method of creating hybrid buprenorphine loaded rifampin/polyethylene glycol/alginate nanoparticles, International journal of biological macromolecules 159 (2020) 204-212. https://doi.org/10.1016/j.ijbiomac.2020.05.065  
  6. M. Khodaei, A. Esmaeili, New and Enzymatic Targeted Magnetic Macromolecular Nanodrug System Which Delivers Methadone and Rifampin Simultaneously, ACS Biomaterials Science & Engineering 6(1) (2019) 246-255. https://doi.org/10.1021/acsbiomaterials.9b01330  
  7. C. Blanco, N.D. Volkow, Management of opioid use disorder in the USA: present status and future directions, The Lancet 393(10182) (2019) 1760-1772. https://doi.org/10.1016/S0140-6736(18)33078-2  
  8. F. Bahramimehr, A. Esmaeili, Producing hybrid nanofiber‐based on/PAN/Fe3O4/zeolite/nettle plant extract/urease and a deformed coaxial natural polymer to reduce toxicity materials in the blood of dialysis patients, Journal of Biomedical Materials Research Part A 107(8) (2019) 1736-1743. https://doi.org/10.1002/jbm.a.36689  
  9. M. Tahmasebi, A. Esmaeili, B. Bambai, New method of identifying morphine in urine samples using nanoparticle-dendrimer-enzyme hybrid system, Arabian Journal of Chemistry 15(2) (2022) 103630. https://doi.org/10.1016/j.arabjc.2021.103630  
  10. E. Ranjbari, A.-A. Golbabanezhad-Azizi, M.R. Hadjmohammadi, Preconcentration of trace amounts of methadone in human urine, plasma, saliva and sweat samples using dispersive liquid-liquid microextraction followed by high performance liquid chromatography, Talanta 94 (2012) 116-122. https://doi.org/10.1016/j.talanta.2012.03.004  
  11. E.A. Álvaro-Alonso, M. Lorenzo, I. Escobar-Rodríguez, A. Aguilar-Ros, Development and validation of a HPLC-UV method for methadone hydrochloride quantification in a new oral solution with preservatives to be implemented in physicochemical stability studies, BMC chemistry 16(1) (2022) 1-10. https://doi.org/10.1186/s13065-022-00827-9  
  12. M. Akhgari, N. Mirahmadi Sani, Z. Mousavi, Determination of Methadone and Tramadol in Vitreous Humor Speci-mens Using Dispersive Liquid Liquid Microextraction and Ultra High Performance Liquid Chromatography, International Jour-nal of Medical Toxicology and Forensic Medicine 11(1) (2021) 31530. https://doi.org/10.32598/ijmtfm.v11i1.31530  
  13. S. Mohammadiazar, F. Hasanli, M. Maham, S. Payami Samarin, Solid‐phase microextraction of methadone in urine samples by electrochemically co‐deposited sol-gel/Cu nanocomposite fiber, Biomedical Chromatography 31(8) (2017) e3926. https://doi.org/10.1002/bmc.3926  
  14. S. Taheri, F. Jalali, N. Fattahi, R. Jalili, G. Bahrami, Sensitive determination of methadone in human serum and urine by dispersive liquid-liquid microextraction based on the solidification of a floating organic droplet followed by HPLC-UV, Journal of separation science 38(20) (2015) 3545-3551. https://doi.org/10.1002/jssc.201500636  
  15. S. Naderi, A. Esmaeili, Preparation of 3D-printed (Cs/PLA/PU) scaffolds modified with plasma and hybridization by Fe@ PEG-CA for treatment of cardiovascular disease, New Journal of Chemistry 44(28) (2020) 12090-12098. https://doi.org/10.1039/D0NJ01430C  
  16. Z. Salarieh, A. Esmaeili, M.H. Pad, Synthesis of cubosomes containing cerium oxide nanoparticles from Lactobacillus acidophilus loaded with glatiramer acetate and carboxymethylcellulose coating, International Journal of Biological Macromolecules (2023) 123215. https://doi.org/10.1016/j.ijbiomac.2023.123215  
  17. B. Abtahi, M. Yousefi, A.A. Kenari, Influence of dietary nucleotides supplementation on growth, body composition and fatty acid profile of Beluga sturgeon juveniles (H uso huso), Aquaculture Research 44(2) (2013) 254-260. https://doi.org/10.1111/j.1365-2109.2011.03028.x  
  18. M.H. Abu Elella, E.A. ElHafeez, E.S. Goda, S. Lee, K.R. Yoon, Smart bactericidal filter containing biodegradable polymers for crystal violet dye adsorption, Cellulose 26(17) (2019) 9179-9206. https://doi.org/10.1007/s10570-019-02698-1  
  19. Z. Adamek, J. Hamačkova, J. Kouril, R. Vachta, I. Stibranyiova, Effect of Ascogen probiotics supplementation on farming success in rainbow trout (Oncorhynchus mykiss) and wels (Silurus glanis) under conditions of intensive culture, Krmiva: Časopis o hranidbi životinja, proizvodnji i tehnologiji krme 38(1) (1996) 11-20.  
  20. F. Adibian, R.S. Ghaderi, Z. Sabouri, J. Davoodi, M. Kazemi, K. Ghazvini, M. Youssefi, S. Soleimanpour, M. Darroudi, Green synthesis of selenium nanoparticles using Rosmarinus officinalis and investigated their antimicrobial activity, BioMetals (2022) 1-12. https://doi.org/10.1007/s10534-021-00356-3  
  21. H. Afsharan, B. Khalilzadeh, H. Tajalli, M. Mollabashi, F. Navaeipour, M.-R. Rashidi, A sandwich type immunosensor for ultrasensitive electrochemical quantification of p53 protein based on gold nanoparticles/graphene oxide, Electrochimica Acta 188 (2016) 153-164. https://doi.org/10.1016/j.electacta.2015.11.133  
  22. N. Aghel, Y. Yamini, A. Hadjiakhoondi, S.M. Pourmortazavi, Supercritical carbon dioxide extraction of Mentha pulegium L. essential oil, Talanta 62(2) (2004) 407-411. https://doi.org/10.1016/j.talanta.2003.08.011  
  23. C. Moore, F. Guzaldo, M. Hussain, D. Lewis, Determination of methadone in urine using ion trap GC/MS in positive ion chemical ionization mode, Forensic science international 119(2) (2001) 155-160. https://doi.org/10.1016/S0379-0738(00)00427-8  
  24. R. Mahmoudi, A. Esmaeili, A. Nematollahzadeh, Formation and investigation of the biology of nanocomposite coated with chitosan-modified oleic acid, Sustainable Chemistry and Pharmacy 32 (2023) 101029. https://doi.org/10.1016/j.scp.2023.101029  
  25. A. Bermejo, C. Remuinan, P. Fernandez, M. Tabernero, GC/MS determination of methadone in urine and plasma from patients under detoxification treatment, Synthesis and Reactivity in Inorganic and Metal-organic Chemistry 31(15) (1998) 2645-2661. https://doi.org/10.1080/00032719808005333  
  26. M. Aliahmadi, A. Esmaeili, Preparation nanocapsules chitosan modified with selenium extracted from the Lactobacillus acidophilus and their anticancer properties, Archives of Biochemistry and Biophysics (2022) 109327. https://doi.org/10.1016/j.abb.2022.109327  
  27. D.-M. Ciucă Anghel, A.-M. Ciobanu, C.M. Guțu, M. Stan, G. Tudor, D.L. Baconi, GC-MS Analysis of Methadone and EDDP in Addicted Patients under Methadone Substitution Treatment: Comparison of Urine and Plasma as Biological Samples, Molecules 27(23) (2022) 8360. https://doi.org/10.3390/molecules27238360  
  28. M. Khodaei, A. Esmaeili, Capsulation of methadone in polymeric layers based on magnetic nanoparticles, Inorganic and Nano-Metal Chemistry 50(4) (2020) 278-285. https://doi.org/10.1080/24701556.2019.1711123  
  29. R.N. Cavalcanti, D.T. Santos, M.A.A. Meireles, Non-thermal stabilization mechanisms of anthocyanins in model and food systems-An overview, Food research international 44(2) (2011) 499-509. https://doi.org/10.1016/j.foodres.2010.12.007  
  30. F.K. Amand, A. Esmaeili, Investigating the properties of electrospun nanofibers made of hybride polymer containing anticoagulant drugs, Carbohydrate polymers 228 (2020) 115397. https://doi.org/10.1016/j.carbpol.2019.115397  
  31. E. Zahedi, A. Esmaeili, N. Eslahi, M.A. Shokrgozar, A. Simchi, Fabrication and characterization of Core-Shell electrospun fibrous Mats containing medicinal herbs for wound healing and skin tissue engineering, Marine drugs 17(1) (2019) 27. https://doi.org/10.3390/md17010027  
  32. A. Esmaeili, S. Niknam, Characterization of nanocapsules containing Elaeagnus angustifolia L. extract prepared using an emulsion-diffusion process, Flavour and Fragrance Journal 28(5) (2013) 309-315. https://doi.org/10.1002/ffj.3164  
  33. A. Esmaeili, S. Niknam, Preparation of polyamide nanocapsules of Elaeagnus angustifolia L. delivery with in vivo studies, Industrial Crops and Products 55 (2014) 49-55. https://doi.org/10.1016/j.indcrop.2014.01.053  
  34. S. Bhattacharjee, DLS and zeta potential-what they are and what they are not?, Journal of controlled release 235 (2016) 337-351. https://doi.org/10.1016/j.jconrel.2016.06.017